Product Name :
ACR polyclonal antibody Background :
Acrosin, a member of the peptidase S1 family, is a major protease present in the acrosome of mature mammalian spermatozoa. Acrosin is a typical serine proteinase with trypsin-like cleavage specificity. The zymogen form, proacrosin, is the precursor of Acrosin synthesized only in the postmeiotic stages of spermatogenesis. The active enzyme functions in the lysis of the zona pellucida, allowing the penetration of sperm through the innermost glycoprotein layers of the ovum. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ACR polyclonal antibody detects endogenous levels of ACR protein. Immunogen :
Synthetic peptide, corresponding to amino acids 1-50 of Human ACR. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of ACR polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug) Lane4:L02 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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