RAPH1 polyclonal antibody 
Datasheet
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Product Name :
RAPH1 polyclonal antibody Background :
Lamellipodin, also called ras-associated and pleckstrin homology domains-containing protein 1 (RAPH1), is a 1302 amino acid member of the MRL family. The peripheral membrane protein mediates localized membrane signals and colocalizes at the tips of filopodia and lamellipodia with ENAH/VASP. Aside from the main isoform, nine additional isoforms have been identified for Lamellipodin (RMO1, RMO1a, RMO1b, RMO1c, RMO1ab, RMO1ac, RMO1bc, RMO1abc and RMO1-RAPH1). RMO1-RAPH1 is expressed in a wide variety of tissues, most highly in brain, heart, ovary and developing embryo. RMO1 is also widely expressed, with highest amounts in liver. Lamellipodin is down regulated in breast and ovarian cancers and shows reduced expression in metastatic osteosarcomas in comparison to primary osteosarcoma tumors. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RAPH1 polyclonal antibody detects endogenous levels of RAPH1 protein. Immunogen :
A synthetic peptide corresponding to residues in Human RAPH1. Conjugate :
Unconjugated Modification :
Unmodification
RAPH1 polyclonal antibody Background :
Lamellipodin, also called ras-associated and pleckstrin homology domains-containing protein 1 (RAPH1), is a 1302 amino acid member of the MRL family. The peripheral membrane protein mediates localized membrane signals and colocalizes at the tips of filopodia and lamellipodia with ENAH/VASP. Aside from the main isoform, nine additional isoforms have been identified for Lamellipodin (RMO1, RMO1a, RMO1b, RMO1c, RMO1ab, RMO1ac, RMO1bc, RMO1abc and RMO1-RAPH1). RMO1-RAPH1 is expressed in a wide variety of tissues, most highly in brain, heart, ovary and developing embryo. RMO1 is also widely expressed, with highest amounts in liver. Lamellipodin is down regulated in breast and ovarian cancers and shows reduced expression in metastatic osteosarcomas in comparison to primary osteosarcoma tumors. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RAPH1 polyclonal antibody detects endogenous levels of RAPH1 protein. Immunogen :
A synthetic peptide corresponding to residues in Human RAPH1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of RAPH1 polyclonal antibody at 1:500 dilution Lane1:HepG2 whole cell lysate Lane2:H9C2 whole cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.