ACE (F940) polyclonal antibody 
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Product Name :
ACE (F940) polyclonal antibody Background :
Angiotensin-converting enzyme (ACE) is a carboxy-terminal dipeptidyl exo-peptidase that converts Angiotensin I to the potent vasopressive hormone, Angiotensin II. There are two isoforms of ACE, the pulmonary ACEP and the testicular ACET. ACEP is a glycoprotein expressed in vascular endothelial cells of the lung, liver, adrenal cortex, pancreas, kidney and spleen. The ACET isoform is expressed exclusively in adult testis by developing sperm cells, specifically, late pachytene spermatocytes. Additionally, ACE inactivates bradykinin, a vasodepressor peptide, and is involved in fluid/electrolyte homeostasis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ACE (F940) polyclonal antibody detects endogenous levels of Angiotensin-converting enzyme protein. Immunogen :
Synthetic peptide, corresponding to amino acids 920-950 of Human ACE. Conjugate :
Unconjugated Modification :
Unmodification
ACE (F940) polyclonal antibody Background :
Angiotensin-converting enzyme (ACE) is a carboxy-terminal dipeptidyl exo-peptidase that converts Angiotensin I to the potent vasopressive hormone, Angiotensin II. There are two isoforms of ACE, the pulmonary ACEP and the testicular ACET. ACEP is a glycoprotein expressed in vascular endothelial cells of the lung, liver, adrenal cortex, pancreas, kidney and spleen. The ACET isoform is expressed exclusively in adult testis by developing sperm cells, specifically, late pachytene spermatocytes. Additionally, ACE inactivates bradykinin, a vasodepressor peptide, and is involved in fluid/electrolyte homeostasis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ACE (F940) polyclonal antibody detects endogenous levels of Angiotensin-converting enzyme protein. Immunogen :
Synthetic peptide, corresponding to amino acids 920-950 of Human ACE. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of ACE (F940) pAb at 1:500 dilution Lane1:PC3 whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:SGC7901 whole cell lysate(40ug) -
Immunohistochemistry (IHC) analyzes of ACE (F940) pAb bin paraffin-embedded human kidney carcinoma tissue at 1:50,showing cytoplasmic and membrane staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
Sex dimorphism in ANGII-mediated crosstalk between ACE2 and ACE in diabetic nephropathy
PMCID: Pubmed No.:29884907
Aortic remodelling induced by obstructive apneas is normalized with mesenchymal stem cells infusion
PMCID: Pubmed No.:31391506
The ACE2 activator diminazene aceturate ameliorates colitis by repairing the gut-vascular barrier in mice
PMCID: Pubmed No.:37127063
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.