RPS4Y1 (K254) polyclonal antibody 
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Product Name :
RPS4Y1 (K254) polyclonal antibody Background :
Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. RPS4Y2 (ribosomal protein S4, Y-linked 2), RPS4Y1 (ribosomal protein S4, Y-linked 1) and RPS4X (ribosomal protein S4, X-linked) are proteins that contain one S4 RNA-binding domain and exist as components of the 40S ribosomal subunit, indicating a possible role in protein synthesis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Ribosomal Protein S4Y1 (K254) polyclonal antibody detects endogenous levels of Ribosomal Protein S4Y1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 225-270 of Human Ribosomal Protein S4Y1. Conjugate :
Unconjugated Modification :
Unmodification
RPS4Y1 (K254) polyclonal antibody Background :
Ribosomes, the organelles that catalyze protein synthesis, are composed of a small subunit (40S) and a large subunit (60S) that consist of over 80 distinct ribosomal proteins. Mammalian ribosomal proteins are encoded by multigene families that contain processed pseudogenes and one functional intron-containing gene within their coding regions. RPS4Y2 (ribosomal protein S4, Y-linked 2), RPS4Y1 (ribosomal protein S4, Y-linked 1) and RPS4X (ribosomal protein S4, X-linked) are proteins that contain one S4 RNA-binding domain and exist as components of the 40S ribosomal subunit, indicating a possible role in protein synthesis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Ribosomal Protein S4Y1 (K254) polyclonal antibody detects endogenous levels of Ribosomal Protein S4Y1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 225-270 of Human Ribosomal Protein S4Y1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of RPS4Y1 (K254) polyclonal antibody at 1:1000 dilution Lane1:HELA whole cell lysate(42ug) Lane2:HEK293T whole cell lysate(37ug) Lane3:LO2 whole cell lysate(38ug) Lane4:RAW264.7 whole cell lysate(57ug)
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.