Product Name :
NY-CO-1 (K914) polyclonal antibody Background :
Protein names: Nuclear export mediator factor NEMF; Serologically defined colon cancer antigen 1; Gene names: NEMF; SDCCAG1. Function: Plays a role in nuclear export. Subunit structure: Interacts (via its N-terminus) with XPO1. Subcellular location: Nucleus.Tissue specificity: Expressed in brain, heart, liver, lung, spleen, and skeletal muscle. Also expressed at lower levels in stomach and testis. Domain: The N-terminal domain contains a nuclear export signal. The C-terminal domain may interact with cargo proteins. Miscellaneous: Active in normal lung tissue, but is inactive in several lung cancer cell lines. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NY-CO-1 (K914) polyclonal antibody detects endogenous levels of NY-CO-1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 881-930 of Human NY-CO-1. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of NY-CO-1 (K914) polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:Raw264.7 whole cell lysate Lane3:PC12 whole cell lysate
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  Immunohistochemistry (IHC) analyzes of NY-CO-1 (K914) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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