Product Name :
ADCY 5/6 (F1052) polyclonal antibody Background :
A cyclase V, also known as ADCY5, is a 1,261 amino acid Adenylyl cyclase that localizes to cellular membranes and contains two guanylate cyclase domains. Similar to other A cyclase proteins, A cyclase V uses magnesium as a cofactor to catalyze the conversion of ATP to cAMP. A cyclase VI, also known as ADCY6 (adenylate cyclase type 6), is a 1,168 amino acid A cyclase that localizes to the membrane and contains two guanylate cyclase domains. Using magnesium as a cofactor, A cyclase VI functions as a calcium-inhibitable A cyclase that catalyzes the conversion of ATP to 3',5'-cyclic AMP and diphosphate and plays a role in a variety of events throughout the body. Multiple isoforms of A cyclase VI exist due to alternative splicing events. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ADCY 5/6 (F1052) polyclonal antibody detects endogenous levels of Adenylate cyclase type 5 and Adenylate cyclase type 6 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 1111-1160 of Human ADCY 5 Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of ADCY 5/6 (F1052) polyclonal antibody at 1:500 dilution Lane1:Panc1 whole cell lysate(40ug) Lane2:PC3 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:Hela whole cell lysate(40ug) Lane5:MCF-7 whole cell lysate(40ug) Lane6:The Pancreas tissue lysate of Mouse(40ug) Lane7:The Pancreas tissue lysate of Rat(40ug)
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  Immunohistochemistry (IHC) analyzes of ADCY 5/6 (F1052) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasm and membrane staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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  Immunohistochemistry (IHC) analyzes of ADCY 5/6 (F1052) pAb in paraffin-embedded human breast carcinoma tissue at 1:50,showing cytoplasm and membrane staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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