ERα (L100) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
ERα (L100) polyclonal antibody Background :
Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Estrogen Receptor-α (L100) polyclonal antibody detects endogenous levels of Estrogen Receptor-α protein. Immunogen :
Synthetic peptide, corresponding to amino acids 61-110 of Human ERα. Conjugate :
Unconjugated Modification :
Unmodification
ERα (L100) polyclonal antibody Background :
Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Estrogen Receptor-α (L100) polyclonal antibody detects endogenous levels of Estrogen Receptor-α protein. Immunogen :
Synthetic peptide, corresponding to amino acids 61-110 of Human ERα. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot (WB) analysis of ERα (L100) polyclonal antibody at 1:500 dillution Lane1:MCF-7 whole cell lysate(40μg) Lane2:NIH-3T3 whole cell lysate(40μg) Lane3:H9C2 whole cell lysate(40μg) -
Immunohistochemistry (IHC) analyzes of ERα (L100) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
Dose-dependent effect of tamoxifen in tamoxifen-resistant breast cancer cells via stimulation by the ERK1/2 and AKT signaling pathways.
PMCID: Pubmed No.:23338782
GPER mediates enhanced cell viability and motility via non-genomic signaling induced by 17β-estradiol in triple-negative breast cancer cells
PMCID: Pubmed No.:24874276
Shugan Liangxue Decoction (舒肝凉血方) down-regulates estrogen receptor α expression in breast cancer cells
PMCID: Pubmed No.:26740221
Shugan Liangxue Decoction (舒肝凉血方) down-regulates estrogen receptor α expression in breast cancer cells
PMCID: Pubmed No.:26740221
Concomitant high expression of ERα36, EGFR and HER2 is associated with aggressive behaviors of papillary thyroid carcinomas
PMCID: Pubmed No.:28947799
Expression of GALNT8 and O-glycosylation of BMP receptor 1A suppress breast cancer cell proliferation by upregulating ERα levels
PMCID: Pubmed No.:34743989
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.