Cyclin E1 (I119) polyclonal antibody 
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Product Name :
Cyclin E1 (I119) polyclonal antibody Background :
Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Cyclin E1 (I119) polyclonal antibody detects endogenous levels of Cyclin E1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 90-135 of Human Cyclin E1. Conjugate :
Unconjugated Modification :
Unmodification
Cyclin E1 (I119) polyclonal antibody Background :
Cyclins were first identified in invertebrates as proteins that oscillate dramatically through the cell cycle. These proteins have been well conserved through evolution and play a critical role in regulation of cell division. cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Cyclin E1 (I119) polyclonal antibody detects endogenous levels of Cyclin E1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 90-135 of Human Cyclin E1. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of Cyclin E1 (I119) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(20ug) Lane2:SK-OVCAR3 whole cell lysate(20ug) Lane3:Hela whole cell lysate(20ug) Lane4:PC12 whole cell lysate(20ug) Lane5:CT26 whole cell lysate(20ug) Lane6:The Testis tissue lysate of Rat(20ug) -
Immunohistochemistry (IHC) analyzes of Cyclin E1 (I119) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
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Selective ligands of estrogen receptor β discovered using pharmacophore mapping and structure-based virtual screening
PMCID: Pubmed No.:25176400
MTA1 downregulation inhibits malignant potential in a small cell lung cancer cell line
PMCID: Pubmed No.:25502548
Water Stress Proteins from Nostoc commune Vauch. Exhibit Anti-Colon Cancer Activities in Vitro and in Vivo
PMCID: Pubmed No.:25524246
PFR peptide, one of the antimicrobial peptides identified from the derivatives of lactoferrin, induces necrosis in leukemia cells
PMCID: Pubmed No.:26860588
ZFX Facilitates Cell Proliferation and Imatinib Resistance in Chronic Myeloid Leukemia Cells
PMCID: Pubmed No.:26912059
Melatonin treatment induces interplay of apoptosis, autophagy, and senescence in human colorectal cancer cells
PMCID: Pubmed No.:24484372
Iso-suillin isolated from Suillus luteus, induces G1 phase arrest and apoptosis in human hepatoma SMMC-7721 cells.
PMCID: Pubmed No.:24606477
Silencing DACH1 Promotes Esophageal Cancer Growth by Inhibiting TGF-β Signaling
PMCID: Pubmed No.:24743895
provides growth and metastasis advantage for human hepatocellular carcinoma
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PCDH18 is frequently inactivated by promoter methylation in colorectal cancer
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SPOP suppresses prostate cancer through regulation of CYCLIN E1 stability
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AIM2 promotes non‐small‐cell lung cancer cell growth through inflammasome‐dependent pathway
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.