Product Name :
TUBA1A (phospho-Y272) polyclonal antibody Background :
Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and ε Tubulin. α and β Tubulins form heterodimers which multimerize to form a microtubule filament. Multiple β Tubulin isoforms (β1, β2, β3, β4, β5, β6 and β8) have been characterized and are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and ε Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas ε Tubulin localizes to the pericentriolar material. ε Tubulin exhibits a cellcycle-specific pattern of localization, first associating with only the older of the centrosomes in a newly duplicated pair and later associating with both centrosomes. Product :
1 mg/ml in Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.3. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
TUBA1A (phospho-Y272) polyclonal antibody detects endogenous levels of TUBA1A protein only when phosphorylated at Try272 . Immunogen :
Synthetic phosphopeptide derived from human TUBA1Aaround the phosphorylation site of Tyrosine272. Conjugate :
Unconjugated Modification :
Phosphorylation

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  Western blot (WB) analysis of TUBA1A (phospho-Y272) polyclonal antibody at 1:500 dilution Lane1:U-87MG whole cell lysate(40ug) Lane2:A375 whole cell lysate(40ug) Lane3:Hela whole cell lysate(40ug) Lane4:A2780 whole cell lysate(40ug) Lane5:EC9706 whole cell lysate(40ug) Lane6:C6 whole cell lysate(40ug) Lane7:CT26 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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