DDDDK-tag (1A8) monoclonal antibody-HRP 
Datasheet
COA
MSDS
SHIP
Product Name :
DDDDK-tag (1A8) monoclonal antibody-HRP Background :
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
DDDDK-tag (1A8) mAb-HRP detects over-expressed or recombinant proteins containing the DDDDK epitope tag. Immunogen :
Synthetic peptide DDDDK-tag. Conjugate :
HRP Modification :
Unmodification
DDDDK-tag (1A8) monoclonal antibody-HRP Background :
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. Product :
1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
DDDDK-tag (1A8) mAb-HRP detects over-expressed or recombinant proteins containing the DDDDK epitope tag. Immunogen :
Synthetic peptide DDDDK-tag. Conjugate :
HRP Modification :
Unmodification
-
Western blot (WB) analysis of DDDDK-tag Mouse Antibody at 1:2000 dilution Lane1:HEK293T whole cell lysate , transfected with HA-DDDDK-p62 48h (3uL) Lane2:HEK293T whole cell lysate(40ug)
Wogonin suppresses human alveolar adenocarcinoma cell A549 migration in inflammatory microenvironment by modulating the IL‐6/STAT3 signaling pathway
PMCID: Pubmed No.:24976450
CARK1 mediates ABA signaling by phosphorylation of ABA receptors
PMCID: Pubmed No.:29928509
Src‐mediated phosphorylation of GAPDH regulates its nuclear localization and cellular response to DNA damage
PMCID: Pubmed No.:32539222
Mutations in spike protein and allele variations in ACE2 impact targeted therapy strategies against SARS-CoV-2
PMCID: Pubmed No.:33738989
Inhibitory Effect of Essential Oil From Fructus of Alpinia zerumbet on Endothelial-to-Mesenchymal Transformation Induced by TGF-β
1 and Downregulation of KLF4
PMCID: Pubmed No.:35794074
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.