Product Name :
Endonuclease G polyclonal antibody Background :
Endonuclease G (EndoG) is a nuclear-encoded mitochondrial nuclease that has been reported to function in apoptosis, DNA recombination and cell proliferation. EndoG is expressed as a precursor protein containing an amino-terminal mitochondrial targeting sequence which is removed when the protein is imported into the mitochondria. During apoptosis EndoG is released from the mitochondria, translocates to the nucleus and cooperates with other nucleases to trigger DNA fragmentation associated with the apoptotic process Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Endonuclease G polyclonal antibody detects endogenous levels of Endonuclease G protein. Immunogen :
Recombinant protein of human Endonuclease G. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of Endonuclease G polyclonal antibody at 1:5000 dilution Lane1:K562 whole cell lysate(40ug) Lane2:U-87MG whole cell lysate(40ug) Lane3:HepG2 whole cell lysate(40ug) Lane4:The Heart tissue lysate of Mouse(40ug) Lane5:The Muscle tissue lysate of RAT(40ug)
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  Immunoprecipitation - Endonuclease G Polyclonal Antibody
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  Immunoprecipitation - Endonuclease G Polyclonal Antibody
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  Immunoprecipitation - Endonuclease G Polyclonal Antibody

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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