Product Name :
PPM1K polyclonal antibody Background :
PP2Cκ (protein phosphatase 2C isoform κ), also known as PP2C-type mitochondrial phosphoprotein phosphatase and PP2C domain-containing protein phosphatase 1K, is a 372 amino acid mitochondrial matrix protein that regulates the opening of mitochondrial membrane permeability transition pores. PP2Cκ is essential for cell survival, cardiac function and embryonic development. Knockdown of PP2Cκ results in cell death due to loss of mitochondrial membrane potential. PP2Cκ specifically binds to the branched-chain-αketoacid dehydrogenase (BCKD) complex and induces dephosphorylation of Ser293, effectively leading to the inhibition of branched chain amino acid metabolism. Highest expression of PP2Cκ is found in brain, diaphragm and heart. There are three isoforms of PP2Cκ that are produced as a result of alternative splicing events. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4. Storage&Stability :
Store at 4°C short term. Aliquot and store at -25°C long term. Avoid freeze-thaw cycles. Specificity :
PPM1K polyclonal antibody detects endogenous levels of PPM1K protein. Immunogen :
Synthetic peptide, corresponding to Human PPM1K. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot (WB) analysis of PPM1K polyclonal antibody at 1:500 dilution LaneA:A549 whole cell lysate LaneB:H1792 whole cell lysate LaneC:CT-26 whole cell lysate LaneD:PC12 whole cell lysate

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---