Product Name :
LEF-1 (F38) polyclonal antibody Background :
Transcription factor that binds DNA in a sequence-specific manner.Participates in the Wnt signaling pathway (By similarity).Activates transcription of target genes in the presence of CTNNB1 and EP300 (By similarity).PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity).TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1.Regulates T-cell receptor alpha enhancer function.Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression (By similarity).May play a role in hair cell differentiation and follicle morphogenesis (By similarity). Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
LEF-1 polyclonal antibody detects endogenous levels of LEF-1 protein. Immunogen :
Synthetic peptide, corresponding to Human LEF-1. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of LEF-1 polyclonal antibody at 1:1000 dilution Lane1:The brain tissue lysate of Mouse(30ug) Lane2:The brain tissue lysate of Rat(30ug) Lane3:HuT78 whole cell lysate(30ug) Lane4:myla2059 whole cell lysate(30ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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