FoxO3A (P247) polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
FoxO3A (P247) polyclonal antibody Background :
FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. Forkhead transcription factors of the FoxO subfamily are emerging as a shared component among pathways regulating diverse cellular functions, such as differentiation, metabolism, proliferation, and survival. Their transcriptional output is controlled via a two-tiered mechanism of phosphorylation and acetylation.FOXO3A is a transcriptional activator which triggers apoptosis in the absence of survival factors. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
FoxO3A (P247) polyclonal antibody detects endogenous levels of FoxO3A protein. Immunogen :
Synthetic peptide, corresponding to amino acids 221-270 of Human FoxO3A. Conjugate :
Unconjugated Modification :
Unmodified
FoxO3A (P247) polyclonal antibody Background :
FOXO3A belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. Forkhead transcription factors of the FoxO subfamily are emerging as a shared component among pathways regulating diverse cellular functions, such as differentiation, metabolism, proliferation, and survival. Their transcriptional output is controlled via a two-tiered mechanism of phosphorylation and acetylation.FOXO3A is a transcriptional activator which triggers apoptosis in the absence of survival factors. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
FoxO3A (P247) polyclonal antibody detects endogenous levels of FoxO3A protein. Immunogen :
Synthetic peptide, corresponding to amino acids 221-270 of Human FoxO3A. Conjugate :
Unconjugated Modification :
Unmodified
-
Western blot (WB) analysis of FoxO3A (P247) polyclonal antibody at 1:1000 dilution Lane1:A549 whole cell lysate(30ug) Lane2:SP2/0 whole cell lysate(30ug) Lane3:C6 whole cell lysate(30ug) -
Immunohistochemistry (IHC) analyzes of FoxO3A (P247) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. -
Immunohistochemistry (IHC) analyzes of FoxO3A (P247) pAb in paraffin-embedded human breast carcinoma tissue at 1:100. -
Immunohistochemistry (IHC) analyzes of FoxO3A (P247) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.
MicroRNA-155 Is Involved in the Pathogenesis of Ulcerative Colitis by Targeting FOXO3a
PMCID: Pubmed No.:24583476
HDAC2 Selectively Regulates FOXO3a-Mediated Gene Transcription during Oxidative Stress-Induced Neuronal Cell Death.
PMCID: Pubmed No.:25609639
Role of the AMPK signaling pathway in early brain injury after subarachnoid hemorrhage in rats
PMCID: Pubmed No.:25697836
Foxc2 enhances proliferation and inhibits apoptosis through activating Akt/mTORC1 signaling pathway in mice pre-adipocyte
PMCID: Pubmed No.:26113535
Expression of Transcription Factor FOXO3a is Decreased in Patients with Ulcerative Colitis
PMCID: Pubmed No.:26481742
Expression of Transcription Factor FOXO3a is Decreased in Patients with Ulcerative Colitis
PMCID: Pubmed No.:26481742
MicroRNA-155 Is Involved in the Pathogenesis of Ulcerative Colitis by Targeting FOXO3a
PMCID: Pubmed No.:24583476
SIRT2-mediated FOXO3a deacetylation drives its nuclear translocation triggering FasL-induced cell apoptosis during renal ischemia reperfusion
PMCID: Pubmed No.: 28078537
Foxc2 enhances proliferation and inhibits apoptosis through activating Akt/mTORC1 signaling pathway in mouse preadipocytes
PMCID: Pubmed No.:26113535
Ketogenic Metabolism Inhibits Histone Deacetylase (HDAC) and Reduces Oxidative Stress After Spinal Cord Injury in Rats
PMCID: Pubmed No.:29024787
Expression of Transcription Factor FOXO3a is Decreased in Patients with Ulcerative Colitis
PMCID: Pubmed No.:26481742
Role of the AMPK signaling pathway in early brain injury after subarachnoid hemorrhage in rats
PMCID: Pubmed No.:25697836
Expression of PH Domain Leucine-rich Repeat Protein Phosphatase, Forkhead Homeobox Type O 3a and RAD51, and their Relationships with Clinicopathologic Features and Prognosis in Ovarian Serous Adenocarcinoma
PMCID: Pubmed No.:28139510
GL-V9, a new synthetic flavonoid derivative, ameliorates DSS-induced colitis against oxidative stress by up-regulating Trx-1 expression via activation of AMPK/FOXO3a pathway
PMCID: Pubmed No.:26327408
LZ-101, a novel derivative of danofloxacin, induces mitochondrial apoptosis by stabilizing FOXO3a via blocking autophagy flux in NSCLC cells
PMCID: Pubmed No.:31217472
An Integrated Proteomics and Bioinformatics Approach Reveals the Anti-inflammatory Mechanism of Carnosic Acid
PMCID: Pubmed No.:29713284
Follicle loss and PTEN/PI3K/mTOR signaling pathway activated in LepR-mutated mice
PMCID: Pubmed No.:30145913
FOXO3 Inhibits Human Gastric Adenocarcinoma (AGS) Cell Growth by Promoting Autophagy in an Acidic Microenvironment
PMCID: Pubmed No.:30138933
Circular RNA circFoxo3 Promotes Granulosa Cell Apoptosis Under Oxidative Stress Through Regulation of FOXO3 Protein
PMCID: Pubmed No.:36394459
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.