Product Name :
Bioepitope® Bradford Protein Assay Kit Applications :
1. Make Biogot Coomassie Bradford Assay Reagent return to RT before use, and mix well. 2. Please wear the lab coat and gloves to operate. 3. Makesure the absorbency at 595nm within the range of the standard curve. 4. Good linear range for samples is from 50-2000μg/ml. Background :
Bradford coomassie-binding, colorimetric method for total protein quantitation is one of dye binding methods.Coomassie Briliant Blue G-250 in the free state shows brown, and its maximum light absorbance is 488nm; When Coomassie Briliant Blue G-250 combines with protein ,it turns brown into blue,and the maximum absorption is 595 nm. The absorbance at 595nm is proportional to the protein concentrations.Protein binding Coomassie Briliant BlueG250 reaches a balance in about 2 mins.The complex remains stable in 1 hour at room temperature. The method has superiority such as simple and convenient operation, sensitive reaction (4 times higher than Lowry Method), etc. Biogot Bradford protein assay kit is developed based on Bradford coomassie-binding method.This method has traits of simple and convenient operation，high sensitivity，accurate quantification and stable effect. Alternative Name :
Classification :
Protein Related Reagents Format :
Kit Contents :
Biogot Coomassie Bradford Assay Reagent：100ml，stored at 4℃。 Bovine Serum Albumin Standard: 100 mg (Power), Stored at 4℃.(Recommend to make stock solution(5mg/ml) and aliquot before store at 4℃ ) Storage & Shelf Life :
Store at 4° C Procedure :
A) Standard Preparation: Dilute stock solution from 5mg/ml to 0.5mg/ml. The diluent used should be the same as used for the protein samples.Label 7 test tubes with A-G and prepare the standards as indicated below. The following dilutions are suitable for duplicate Standard assays. B) Micro-plate Procedure (Sample to WR ratio = 1:20): 1. Pipette each standard or unknown sample into the appropriate microplate wells.The sample would be less than 20μl,and add diluent up to 20 μl. 2. Add 200μL of the Biogot Coomassie Bradford Assay Reagent to each well and mix with plate shaker for 30 seconds. 3. Cover plate and incubate for 5 minutes at RT. 4. Measure the absorbency at or near 595 nm on a plate reader. 5. Use the standard curve to calculate the protein concentration of each unknown sample. Research use :
For research use only, not for use in diagnostic procedures. Expiration Date :
Six months from date of reconstitution.

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Blocking peptide available as BD0029P