Product Name :
Caspase 9 (Tyr153) polyclonal antibody Background :
Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Caspase 9 (Tyr153) polyclonal antibody detects endogenous levels of full length caspase-9 (48 kDa) and large fragments of caspase-9 (35 kDa and 37kDa). Immunogen :
Synthetic peptide, corresponding to amino acids 130-180 of Human Caspase 9. Conjugate :
Unconjugated Modification :
Unmodified
Caspase 9 (Tyr153) polyclonal antibody Background :
Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. This caspase is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 has been reported to be approximately 47 kD. This caspase is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
Caspase 9 (Tyr153) polyclonal antibody detects endogenous levels of full length caspase-9 (48 kDa) and large fragments of caspase-9 (35 kDa and 37kDa). Immunogen :
Synthetic peptide, corresponding to amino acids 130-180 of Human Caspase 9. Conjugate :
Unconjugated Modification :
Unmodified
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Western blot (WB) analysis of Caspase 9 (Tyr153) polyclonal antibody at 1:500 dilution Lane1:LO2 whole cell lysate(40ug) Lane2:L02 treated with PBS(1×PBS,PH7.4) for 30 minutes whole cell lysate(40ug) Lane3:L02 treated with PBS(1×PBS,PH7.4) for 120 minutes whole cell lysate(40ug) Lane4:L02 treated with PBS(1×PBS,PH7.4) for 240 minutes whole cell lysate(40ug)
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Inhibitory effect of epirubicin-loaded lipid microbubbles with conjugated anti-ABCG2 antibody combined with therapeutic ultrasound on multiple myeloma cancer stem cells.
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Gambogic acid enhances the radiosensitivity of human esophageal cancer cells by inducing reactive oxygen species via targeting Akt/mTOR pathway
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High glucose concentration induces endothelial cell proliferation by regulating cyclin‐D2‐related miR‐98
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Curdione Inhibits Proliferation of MCF-7 Cells by Inducing Apoptosis
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Norisoboldine induces apoptosis of fibroblast-like synoviocytes from adjuvant-induced arthritis rats
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High glucose concentration induces endothelial cell proliferation by regulating cyclin‐D2‐related miR‐98
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Foxc2 enhances proliferation and inhibits apoptosis through activating Akt/mTORC1 signaling pathway in mouse preadipocytes
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FXR antagonism of NSAIDs contributes to drug-induced liver injury identified by systems pharmacology approach
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Inhibitory effect of epirubicin-loaded lipid microbubbles with conjugated anti-ABCG2 antibody combined with therapeutic ultrasound on multiple myeloma cancer stem cells
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Tan IIA inhibits H1299 cell viability through the MDM4‑IAP3 signaling pathway
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Anti-proliferative potential of Glucosamine in renal cancer cells via inducing cell cycle arrest at G0/G1 phase
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Bortezomib enhances the radiosensitivity of hypoxic cervical cancer cells by inhibiting HIF-1α expression
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HnRNP-L mediates bladder cancer progression by inhibiting apoptotic signaling and enhancing MAPK signaling pathways
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3B, a novel of photosensitizer, exhibited anti-tumor effects via mitochondrial apoptosis pathway in MCF-7 human breast carcinoma cells
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Curdione Inhibits Proliferation of MCF-7 Cells by Inducing Apoptosis
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Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.