Contact us : info@bioworlde.com
Home > Product > Primary Antibodies
EpCAM polyclonal antibody BS6213
  • Western blot analysis of EpCAM expression in HCT116 (A), CT26 (B), PC12 (C) whole cell lysates.
  • Immunofluorescent analysis of EpCAM staining in HepG2 cells.
Product NameEpCAM polyclonal antibody
Catalog No.BS6213
Swiss-ProtP16422
Host Rabbit
ReactivityHuman, Mouse, Rat
ApplicationsWB, IF/ICC
Application_allWB,1:500 - 1:1000|IF/ICC,1:50 - 1:200
BiowMW~ 40 kDa
Alternative NameEPCAM;DIAR5;EGP-2;EGP314;EGP40;ESA;HNPCC8;KS1/4;KSA;M4S1;MIC18;MK-1;TACSTD1;TROP1
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodified
Browse similar products>>
Size Price
50ul $208
100ul $358
Add to cart My orders
Product Name :
EpCAM polyclonal antibody
Background :
This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
EpCAM polyclonal antibody detects endogenous levels of EpCAM protein.
Immunogen :
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EpCAM.
Conjugate :
Unconjugated
Modification :
Unmodified
  • Western blot analysis of EpCAM expression in HCT116 (A), CT26 (B), PC12 (C) whole cell lysates.
  • Immunofluorescent analysis of EpCAM staining in HepG2 cells.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
COPYRIGHT © 2015-2018 Bioworld Technology, Inc. All rights reserved POLYCLONAL AND MONOCLONAL ANTIBODY CENTER