Product Name :
VPS34 polyclonal antibody Background :
Phosphatidylinositol 3-kinases (PI3Ks) phosphorylate the 3' OH position of the inositol ring of inositol lipids. PI 3-kinase p100 (phosphoinositide-3-kinase p100 subunit), also known as hVps34 or PIK3C3 (phosphoinositide-3-kinase, class III), is a member of the PI3/PI4-kinase family. It is ubiquitously expressed with predominant expression in skeletal muscle and is believed to participate in endosome to lysosome transport, multivesicular body formation, autophagy and retrograde endosome to Golgi transport. PI 3-kinase p100 is the catalytic subunit of class III PI3Ks and forms a heterodimer with p150, a regulatory subunit of class 3 PI3Ks. PI 3-kinase p100 exclusively phosphorylates phosphatidylinositol to produce PtdIns3P. Unlike class I PI3Ks, whose activities are enhanced in the presence of magnesium, PI 3-kinase p100 activity is enhanced by manganese. Its activity can also be regulated by nutrients, suggesting an important role of PI-3 kinase p100 in the regulation of mTOR protein synthesis and autophagy. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
VPS34 polyclonal antibody detects endogenous levels of VPS34 protein. Immunogen :
recombinant protein Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of PI 3 Kinase Class 3 on C2C12 cells lysates using anti-PI 3 Kinase Class 3 antibody at 1/1,000 dilution.
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  Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PI 3 Kinase Class 3 antibody. Counter stained with hematoxylin.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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