Product Name :
Tle6 polyclonal antibody Background :
The Notch signaling pathway controls various cellular interactions that are important for the specification of a variety of fates in both vertebrates and invertebrates. Key players in the Notch pathway are the TLE genes (for transducin-like enhancer of split, also designated ESG for enhancer of split groucho), which are human homologs of the Drosophila groucho gene. TLE6 (transducin-like enhancer of split 6), also known as GRG6, is a 449 amino acid cytoplasmic protein belonging to the WD repeat groucho/TLE family. As a member of the subcortical maternal complex (SCMC), TLE6 is essential for zygotes to progress beyond the first embryonic cell divisions. TLE6 contains seven WD repeats, a motif known to mediate protein-protein interactions. The WD40 repeat family of proteins is suggested to be involved in signal transduction, RNA processing, gene regulation, vesicular trafficking, cytoskeletal assembly and may play a role in the control of cytotypic differentiation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles. Specificity :
Tle6 polyclonal antibody detects endogenous levels of Tle6 protein. Immunogen :
Synthetic peptide within mouse Tle6 aa 150-200. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of Tle6 on different lysates using anti-Tle6 antibody at 1/500 dilution. Positive control： Lane1: Mouse ovary tissue Lane2: HepG2
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  ICC staining Tle6 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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