Product Name :
SERPINA1 polyclonal antibody Background :
Cumulative damage to lung tissue by Neutrophil Elastase is responsible for the development of pulmonary emphysema, an irreversible lung disease characterized by loss of lung elasticity. a 1-antitrypsin (AAT), a 394 amino acid hepatic acute phase protein, predominantly inhibits Neutrophil Elastase. AAT is highly expressed in liver and in cultured hepatoma cells and, to a lesser extent, in macrophages. AAT is a highly polymorphic glycosylated serum protein with characteristic isoelectric-focusing patterns for most variants. The gene encoding AAT maps to a region of human chromosome 14 that includes a related serine protease inhibitor (serpin) gene which encodes corticosteroid-binding globulin. Oxidation of the methionine 358 residue in the active center of AAT results in a dramatic decrease in inhibitory activity towards elastase. AAT also has a moderate affinity for plasmin and Thrombin. AAT deficiency is associated with a 20-30 fold increased risk of precocious pulmonary emphysema. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
SERPINA1 polyclonal antibody detects endogenous levels of SERPINA1 protein. Immunogen :
recombinant protein Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of alpha 1 Antitrypsin on human spleen lysates using anti-alpha 1 Antitrypsin antibody at 1/1,000 dilution.
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  ICC staining alpha 1 Antitrypsin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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