Product Name :
PPP1R1A polyclonal antibody Background :
The inhibitor of protein phosphatase 1 (IPP-1, I-1) plays a role in regulating the phosphorylation of other proteins, and is itself phosphorylated by a cyclic AMP-dependent protein kinase. IPP-1 is present in skeletal muscles and in distinct neuronal systems of the brain. The localization and expression of IPP-1 suggests that it may play discrete roles in certain regions and developing stages of the brain, independent of the regulation of protein phosphatase type 1 (PP-1). PP-1 binds to both phosphorylated and dephosphorylated IPP-1. Conversion of PP-1 to an Mn2+-dependent state appears to play a role in its regulation by IPP-1. IPP-1 attenuates the activity of glycogen phosphorylase and is thought to play an important role in the hormonal control of glycogen metabolism. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
PPP1R1A polyclonal antibody detects endogenous levels of PPP1R1A protein. Immunogen :
recombinant protein Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of PPP1R1A on Rat brain lysates using anti-PPP1R1A antibody at 1/1,000 dilution.
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  ICC staining PPP1R1A in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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