Product Name :
PI3K p110δ polyclonal antibody Background :
Phosphatidylinositol 3-kinase (PI 3-kinase) is composed of p85 and p110 subunits. p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85α and p85β), each possessing one SH3 and two SH2 domains. Various p110 forms have been identified. p110α and p110β interact with p85α, and p110α has also been shown to interact with p85β in vitro. It has been shown to bind p85α and β, but it apparently does not phosphorylate these subunits. p110δ has the capacity to autophosphorylate and results in the nearly complete inactivation of the lipid kinase activity. Interestingly, p110γ does not interact with the p85 subunits and has been shown to be activated by α and βγ heterotrimeric G proteins. Two p110δ isoforms have been identified and are widely expressed. Isoform 1 is expressed predominantly in leukocytes while isoform 2 is expressed in normal thymus, lung and spleen tissues. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Specificity :
PI3K p110δ polyclonal antibody detects endogenous levels of PI3K p110δ protein. Immunogen :
Recombinant protein Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of PI 3 Kinase p110 delta on mouse spleen tissue lysate using anti-PI 3 Kinase p110 delta antibody at 1/1,000 dilution.
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  Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PI 3 Kinase p110 delta antibody. Counter stained with hematoxylin.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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