Product Name :
DIAPH1 polyclonal antibody Background :
Dia 1, also known as DIAPH1 (diaphanous homolog 1) or DRF1, a mammalian homolog of the Drosophila diaphanous gene, belongs to a family of formin homology (FH) proteins which are characterized by having tandemly aligned FH1 (formin homology 1) and FH2 (formin homology 2) domains in their carboxy terminal regions. Dia 1 contains a DAD (diaphanous autoregulatory) domain, which is involved in the elongation of actin filaments, and a GBD/FH3 (Rho GTPase-binding/formin homology 3) domain, which interacts with the DAD domain via autoinhibitory interactions to regulate the activation of Dia 1. Dia 1 is required for the assembly of F-actin structures, and regulates the polymerization and depolymerization of actin filaments. Localizing to the cell membrane, Dia 1 is expressed in a wide range of tissues, including brain, heart, lung and kidney. Defects to the gene encoding Dia 1 have been linked to deafness autosomal dominant type 1 (DFNA1), a disorder characterized by sensorineural hearing loss.. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles. Specificity :
DIAPH1 polyclonal antibody detects endogenous levels of DIAPH1 protein. Immunogen :
Recombinant protein corresponding to N-terminal human DIAPH1. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot analysis of DIAPH1 on SiHa cell lysate using anti-DIAPH1 antibody at 1/5,000 dilution.
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  ICC staining DIAPH1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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