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Cullin-3 polyclonal antibody BS90347
  • Western blot analysis of Cullin 3 on different lysates using anti-Cullin 3 antibody at 1/1,000 dilution. Positive control: Lane1: SH-SY5Y Lane2: 293T Lane3: Mouse testis tissue
  • ICC staining Cullin 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Product NameCullin-3 polyclonal antibody
Catalog No.BS90347
Swiss-ProtQ13618(Human) Q9JLV5(Mouse)
Host Rabbit
ReactivityHuman, Mouse
ApplicationsWB IHC FC
Application_allWB:1:500-1:1,000 IHC:1:50-1:200 FC:1:50-1:100
BiowMW89 kDa
Alternative NameCUL-3, CUL3, CUL 3, Cul-3, cul3, CUL3, Cullin-3, Cullin3, KIAA0617, PHA2E,
Purification&PurityPeptide affinity purified
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $258
100ul $398
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Product Name :
Cullin-3 polyclonal antibody
Background :
Cullin proteins comprise a distinct family of mediators that participate in the selective targeting of proteins for ubiquitin (Ub)-mediated proteolysis. CUL-1, which is the mammalian homolog of yeast Cdc53, is an integral component of the E3 ubiquitin ligase complex designated SCF. The SCF (Skp1/CUL-1/F-box protein complex) consists of Skp1 associating with both CUL-1 and an F-box protein, such as Skp2, which determines the substrate specificity of the complex. CUL-1-mediated ubiquitination results in the degradation of cell cycle proteins cyclin D, p21 and cyclin E. Another cullin, CUL-3, facilitates the degradation of cyclin E independent of SCF activity, while CUL-2 associates with the tumor suppressing protein VHL and elongin B to form VBC complexes, which structurally resemble the SCF ligase. Proteolysis also occurs by way of CUL-4 associating with Nedd-8, a ubiquitin-like protein, where it too functions as an active component of a multifunctional E3 complex. CUL-5, also designated vasopressin-activated, calcium-mobilizing protein (VACM-1), is also included in the cullin family as it shares substantial sequence homology with CUL-1.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Specificity :
Cullin-3 polyclonal antibody detects endogenous levels of Cullin-3 protein.
Immunogen :
Peptide.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of Cullin 3 on different lysates using anti-Cullin 3 antibody at 1/1,000 dilution. Positive control: Lane1: SH-SY5Y Lane2: 293T Lane3: Mouse testis tissue
  • ICC staining Cullin 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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