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H2AFY polyclonal antibody BS8670
  • Western blot analysis of extracts of various cell lines, using H2AFY antibody.
  • Western blot analysis of extracts of various cell lines, using H2AFY antibody.
  • Western blot analysis of extracts of various cell lines, using H2AFY antibody.
Product NameH2AFY polyclonal antibody
Catalog No.BS8670
Swiss-ProtO75367
Host Rabbit
ReactivityHuman,Mouse,Rat
ApplicationsWB IHC IF
Application_allWB: 1:500~1:2000 IHC: 1:50~1:200 IF: 1:50~1:200
BiowMW~ 40kDa
Alternative NameCore histone macro-H2A.1; Histone macroH2A1; mH2A1; Histone H2A.y; H2A/y; Medulloblastoma antigen MU-MB-50.205; H2AFY; MACROH2A1
Purification&PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
ConjugateUnconjugated
ModificationUnmodification
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Size Price
50ul $208
100ul $358
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Product Name :
H2AFY polyclonal antibody
Background :
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.
Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :
H2AFY polyclonal antibody detects endogenous levels of H2AFY protein.
Immunogen :
Recombinant full length Human H2AFY.
Conjugate :
Unconjugated
Modification :
Unmodification
  • Western blot analysis of extracts of various cell lines, using H2AFY antibody.
  • Western blot analysis of extracts of various cell lines, using H2AFY antibody.
  • Western blot analysis of extracts of various cell lines, using H2AFY antibody.
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

Price/Size :

USD 368/1mg/vial



Tips: 

For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 


Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.
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