Product Name :
EPHA8 (phospho-Y838) polyclonal antibody Background :
Ephrin type-A receptor 8 is a protein that in humans is encoded by the EPHA8 gene.This gene encodes a member of the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
EPHA8 (phospho-Y838) polyclonal antibody detects endogenous levels of EPHA8 protein only when phosphorylated at Tyr838. Immunogen :
Synthetic phosphopeptide derived from human EPHA8 around the phosphorylation site of Tyrosine 838. Conjugate :
Unconjugated Modification :
Phosphorylation

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  Western blot (WB) analysis of EPHA8 (phospho-Y838) polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:The Spleen tissue lysate of Mouse(40ug) Lane3:The Kidney tissue lysate of Rat(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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