Product Name :
KLF13 (Acetyl-K166) polyclonal antibody Background :
KLF13 belongs to a family of transcription factors thatcontain 3 classical zinc finger DNA-binding domains consisting of azinc atom tetrahedrally coordinated by 2 cysteines and 2 histidines(C2H2 motif). These transcription factors bind to GC-rich sequencesand related GT and CACCC boxes. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
KLF13 (Acetyl-K166) polyclonal antibodydetects endogenous levels of KLF13 protein when Acetyl at K166. Immunogen :
Synthetic peptide including acetyl-lysine 166 of KLF13. Conjugate :
Unconjugated Modification :
Acetylated

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  Western blot (WB) analysis of KLF13 (Acetyl-K166) polyclonal antibodyat 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:Jurkat whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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