Product Name :
NDFIP1 polyclonal antibody Background :
Nedd4 family interacting protein-1 (Ndfip1), also referred to as N4WBP5, is a member of a family of highly conserved proteins. It has three transmembrane domains in its carboxy terminal and two PY motifs in its amino terminal. Ndfip1 is a 221 amino acid, Golgi-associated protein that may play a role in Golgi structure and function. Ndfip1 binds the WW domains of a number of Nedd4 family members called HECT-type E3 ubiquitin ligases. Ndfip1 is strongly expressed in surviving neurons around a site of injury. This suggests that ubiquitination may be a possible survival strategy and Ndfip1 may act as a neuroprotective protein. Ndfip1 may also have an effect on the function of Itch, another E3 ubiquitin ligase. Expression of Ndfip1 and its association with Itch may be promoted by T cell activation. Mice lacking Ndfip1 exhibit inactivation of Itch and accumulation of JunB causing them to die prematurely with severe skin and lung inflammation. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NDFIP1 polyclonal antibody detects endogenous levels of NDFIP1 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 170-220 of Human NDFIP1. Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot (WB) analysis of NDFIP1 polyclonal antibody at 1:500 dilution Lane1:C6 whole cell lysate(40ug) Lane2:AML-12 whole cell lysate(40ug) Lane3:HepG2 whole cell lysate(40ug) Lane4:HEK293T whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---