Product Name :
USP36 polyclonal antibody Background :
Human USP36, encoding a DUB enzyme, was isolated from ovarian cancer cells using RT-PCR and characterized. Identified DUB enzyme activity of USP36 by analyzing its capability to cleave the ubiquitin. Structural and immunoprecipitation analyses revealed for the first time that USP36 contains the PEST motif and is polyubiquitinated. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
USP36 polyclonal antibody detects endogenous levels of USP36 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 500-550 of Human USP36. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of USP36 polyclonal antibody at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:PC12 whole cell lysate(40ug) Lane4:3T3-L1 whole cell lysate(40ug) Lane5:BV2 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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