Product Name :
TUBA3C/TUBA3D/TUBA3E polyclonal antibody Background :
Tubulin is a major cytoskeleton component that has five distinct forms, designated α, β, γ, δ and ε Tubulin. α and β tubulins form heterodimers which multimerize to form a microtubule filament. There are 5 β Tubulin isoforms (β1, β2, β3, β4A and β4B) that are expressed in mammalian tissues. β1 and β4 are present throughout the cytosol, β2 is present in the nuclei and nucleoplasm, and β3 is a neuron-specific cytoskeletal protein. γ Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both δ Tubulin and ε Tubulin are associated with the centrosome. δ Tubulin is a homolog of the Chlamydomonas δ Tubulin Uni3 and is found in association with the centrioles, whereas ε Tubulin localizes to the pericentriolar material. ε Tubulin exhibits a cell cycle-specific pattern of localization; first associating with only the older of the centrosomes in a newly duplicated pair, and later associating with both centrosomes. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
TUBA3C/TUBA3D/TUBA3E polyclonal antibody detects endogenous levels of TUBA3C/TUBA3D/TUBA3E protein. Immunogen :
Synthetic peptide, corresponding to amino acids 400-450 of Human TUBA3C. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of TUBA3C/TUBA3D/TUBA3E polyclonal antibody at 1:500 dilution Lane1:The Brain tissue lysate of Rat(40ug) Lane2:The Brain tissue lysate of Mouse(40ug) Lane3:THP-1 whole cell lysate(40ug) Lane4:K562 whole cell lysate(40ug) Lane5:Hela whole cell lysate(40ug) Lane6:The Testis tissue lysate of Rat(40ug) Lane7:The Testis tissue lysate of Mouse(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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