NEK5 polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
NEK5 polyclonal antibody Background :
The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. Nek5 (NimA-related protein kinase 5) is a 708 amino acid protein that is related to NIMA, a protein that was originally discovered in Aspergillus nidulans and is necessary for entry into mitosis. One of several members of the Ser/Thr protein kinase super family, Nek5 contains one protein kinase domain through which it catalyzes the ATP-dependent phosphorylation of target proteins. Like NIMA, Nek5 may be involved in mitotic regulation and cell cycle control. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NEK5 polyclonal antibody detects endogenous levels of NEK5 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 150-200 of Human NEK5. Conjugate :
Unconjugated Modification :
Unmodification
NEK5 polyclonal antibody Background :
The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. Nek5 (NimA-related protein kinase 5) is a 708 amino acid protein that is related to NIMA, a protein that was originally discovered in Aspergillus nidulans and is necessary for entry into mitosis. One of several members of the Ser/Thr protein kinase super family, Nek5 contains one protein kinase domain through which it catalyzes the ATP-dependent phosphorylation of target proteins. Like NIMA, Nek5 may be involved in mitotic regulation and cell cycle control. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NEK5 polyclonal antibody detects endogenous levels of NEK5 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 150-200 of Human NEK5. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of NEK5 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:K562 whole cell lysate(40ug) Lane3:C0S-7 whole cell lysate(40ug)
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.