POLR3B polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
POLR3B polyclonal antibody Background :
RNA polymerase II (Pol II) is a multi-subunit complex responsible for catalyzing the transcription of DNA into RNA. POLR3B (polymerase (RNA) III (DNA directed) polypeptide B), also known as C128 or RPC2, is a 1,133 amino acid protein that localizes to the nucleus and belongs to the RNA polymerase β chain family. Existing as the second largest component of the multi-protein RNA polymerase III (Pol III) complex, POLR3B functions as a DNA-dependent RNA polymerase that specifically catalyzes the transcription of DNA into RNA using ribonucleoside triphosphates as substrates. The gene encoding POLR3B maps to human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome. Chromosome 12 is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and Trisomy 12p, which causes facial developmental defects and seizure disorders. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
POLR3B polyclonal antibody detects endogenous levels of POLR3B protein. Immunogen :
Synthetic peptide, corresponding to amino acids 330-380 of Human POLR3B. Conjugate :
Unconjugated Modification :
Unmodification
POLR3B polyclonal antibody Background :
RNA polymerase II (Pol II) is a multi-subunit complex responsible for catalyzing the transcription of DNA into RNA. POLR3B (polymerase (RNA) III (DNA directed) polypeptide B), also known as C128 or RPC2, is a 1,133 amino acid protein that localizes to the nucleus and belongs to the RNA polymerase β chain family. Existing as the second largest component of the multi-protein RNA polymerase III (Pol III) complex, POLR3B functions as a DNA-dependent RNA polymerase that specifically catalyzes the transcription of DNA into RNA using ribonucleoside triphosphates as substrates. The gene encoding POLR3B maps to human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome. Chromosome 12 is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and Trisomy 12p, which causes facial developmental defects and seizure disorders. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
POLR3B polyclonal antibody detects endogenous levels of POLR3B protein. Immunogen :
Synthetic peptide, corresponding to amino acids 330-380 of Human POLR3B. Conjugate :
Unconjugated Modification :
Unmodification
-
Western blot (WB) analysis of POLR3B polyclonal antibody at 1:500 dilution Lane1:CT26 whole cell lysate(20ug) Lane2:H9C2 whole cell lysate(20ug) Lane3:U-87MG whole cell lysate(40ug) Lane4:HCT116 whole cell lysate(40ug) Lane5:MCF-7 whole cell lysate(40ug)
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.