Product Name :
RNF138 polyclonal antibody Background :
The RING-type zinc finger motif is present in a number of viral and eukaryotic proteins and is made of a conserved cysteine-rich domain that is able to bind two zinc atoms. Proteins that contain this conserved domain are generally involved in the ubiquitination pathway of protein degradation. RNF13 (ring finger protein 13), also known as RZF, FLJ93817 or MGC13689, is a novel 381 amino acid E3 ubiquitin ligase that localizes to the nucleus. RNF13 contains one RING-type zinc finger and the C-terminal portion of RNF13 has the ability to mediate ubiquitination. Recent studies suggest that RNF13 may be involved in the development of pancreatic cancer via ubiquitin-mediated modification of proteins. The gene encoding RNF13 maps to human chromsome 3q25.1, and a pseudogene (which is also located on chromosome 3), exists for this gene. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RNF138 polyclonal antibody detects endogenous levels of RNF138 protein. Immunogen :
Synthetic peptide, corresponding Human RNF138. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of RNF138 polyclonal antibody at 1:500 dilution Lane1:CT26 whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:COS-7 whole cell lysate(40ug) Lane4:MCF-7 whole cell lysate(40ug) Lane5:A549 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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