Product Name :
MRPL15 polyclonal antibody Background :
Mammalian mitochondrial ribosomes (mitoribosomes) are responsible for protein synthesis within the mitochondrion. The mitoribosomes are composed of a 4:1 ratio of protein to RNA, with the proteins forming two subunits, the 28S subunit and the 39S subunit. Across species, the proteins that make up the mitoribosome subunits vary greatly in sequence, preventing easy recognition by sequence homology. MRP-L15 (mitochondrial 39S ribosomal protein L15), also known as L15mt, is a 296 amino acid mitochondrial ribosomal protein belonging to the ribosomal protein L15P family. Localized to mitochondria, MRP-L15 is present in the 39S subunit of the mitoribosomes. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
MRPL15 polyclonal antibody detects endogenous levels of MRPL15 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 191-235 of Human MRPL15. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of MRPL15 polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:786-O whole cell lysate(40ug) Lane4:The heart tissue lysate of Mouse(40ug) Lane5:The heart tissue lysate of Rat(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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