Product Name :
MRPL46 polyclonal antibody Background :
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated protein (Cas9) system is an adaptive immune response defense mechanism used by archea and bacteria for the degradation of foreign genetic material. This mechanism can be repurposed for other functions, including genomic engineering for mammalian systems, such as gene knockout (KO). CRISPR/Cas9 KO Plasmid products enable the identification and cleavage of specific genes by utilizing guide RNA (gRNA) sequences derived from the Genome-scale CRISPR Knock-Out (GeCKO). Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
MRPL46 polyclonal antibody detects endogenous levels of MRPL46 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 114-158 of Human MRPL46. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of MRPL46 polyclonal antibody at 1:500 dilution Lane1:Hela whole cell lysate(40ug) Lane2:A549 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug) Lane5:RAW264.7 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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