Product Name :
COX4I2 polyclonal antibody Background :
The cytochrome c oxidase (COX) family of proteins function as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water. The mammalian COX apoenzyme is a dimer, with each monomer consisting of 13 subunits, some of which are mitochondrial and some of which are nuclear. Cytochrome c oxidase IV (COX4) is a nuclear-encoded subunit of COX that may play a role in regulating COX activity. COX4 is expressed ubiquitously in adult human tissue with the strongest levels of expression in the pancreas and moderate expression levels in heart, skeletal muscle and placenta. Two isoforms exist for COX4, namely COX4I1 and COX4I2, and they are encoded by two different genes. COX4I2 (Cytochrome c oxidase subunit 4 isoform 2), also known as COX4B, COX4-2, COX4L2 or COXIV-2, is the less common isoform and is incorporated into the COX apoenzyme under hypoxic conditions to optimize COX activity. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
COX4I2 polyclonal antibody detects endogenous levels of COX4I2 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 77-121 of Human COX4I2. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of COX4I2 polyclonal antibody at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:Hcc827 whole cell lysate(40ug) Lane3:The lung tissue lysate of Fetal Mouse(40ug) Lane4:The lung tissue lysate of Fetal Rat(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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