Product Name :
DDX54 polyclonal antibody Background :
DEAD-box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure and ribosome/spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX54 (DEAD polypeptide 54), also known as DP97, is an 881 amino acid protein that contains 2 bipartite nuclear localization signals, 3 nuclear receptor boxes (LXXLL motifs), a potential CoRNR box, and several stretches of glutamate and lysine residues. DDX54 is ubiquitously expressed, with highest expression in pancreas and lung. DDX54 co-localizes with ERα to structures in the nucleoplasm. DDX54 represses ERα transcriptional activity and acts as a nuclear receptor co-repressor against ERβ, progesterone, glucocorticoid and RARα Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
DDX54 polyclonal antibody detects endogenous levels of DDX54 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 602-646 of Human DDX54 Conjugate :
Unconjugated Modification :
Unmodification

• 
  Western blot (WB) analysis of DDX54 polyclonal antibody at 1:500 dilution Lane1:HCC827 whole cell lysate(40ug) Lane2:L02 whole cell lysate(40ug) Lane3:HEK293T whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

---

Price/Size :

USD 368/1mg/vial

---

---

Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

---

Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

---

Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

---

Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

---

Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

---