CIDEB polyclonal antibody 
Datasheet
COA
MSDS
SHIP
Product Name :
CIDEB polyclonal antibody Background :
The DNA fragmentation factor (DFF) is involved in the caspase-3 apoptotic pathway. DFF is composed of two subunits, a 45 kDa subunit called DFF-45 (also designated ICAD, for inhibitor of CAD), and CPAN (caspase-activated nuclease), also designated CAD (caspase-activated deoxyribonuclease). CPAN is a DNase that is responsible for DNA degradation during apoptosis. CPAN is inhibited by DFF-45. Caspase-3 acts to dissociate CPAN from DFF-45, allowing CPAN to enter the nucleus and degrade DNA. CIDE-A and CIDE-B have been identified as proteins that share homology with the N-terminal region of DFF-45. Like CPAN, CIDE-A and CIDE-B promote cell death and DNA fragmentation and are inhibited by DFF-45. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CIDEB polyclonal antibody detects endogenous levels of CIDEB protein. Immunogen :
Synthetic peptide, corresponding to amino acids 104-148 of Human CIDEB Conjugate :
Unconjugated Modification :
Unmodification
CIDEB polyclonal antibody Background :
The DNA fragmentation factor (DFF) is involved in the caspase-3 apoptotic pathway. DFF is composed of two subunits, a 45 kDa subunit called DFF-45 (also designated ICAD, for inhibitor of CAD), and CPAN (caspase-activated nuclease), also designated CAD (caspase-activated deoxyribonuclease). CPAN is a DNase that is responsible for DNA degradation during apoptosis. CPAN is inhibited by DFF-45. Caspase-3 acts to dissociate CPAN from DFF-45, allowing CPAN to enter the nucleus and degrade DNA. CIDE-A and CIDE-B have been identified as proteins that share homology with the N-terminal region of DFF-45. Like CPAN, CIDE-A and CIDE-B promote cell death and DNA fragmentation and are inhibited by DFF-45. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
CIDEB polyclonal antibody detects endogenous levels of CIDEB protein. Immunogen :
Synthetic peptide, corresponding to amino acids 104-148 of Human CIDEB Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of CIDEB polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:SK-OVCAR3 whole cell lysate(40ug) Lane3:The Spleen lysate of Mouse(40ug) Lane4:The Spleen lysate of Rat(40ug)
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.