Product Name :
ZKSCAN5 polyclonal antibody Background :
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. A member of the Krüppel C2H2-type zinc-finger protein family, ZKSCAN5, also known as ZFP95, is a 839 amino acid protein containing thirteen C2H2-type zinc fingers, one KRAB A domain and one SCAN box domain. ZKSCAN5 is located in the nucleus and is highly expressed in testis with lower levels of expression in liver, kidney, brain, striated muscle, lung and heart. Many KRAB A box-containing proteins have been shown to function as transcription repressors, suggesting a possible function of ZKSCAN5. As a result of alternative splicing, three transcripts of ZKSCAN5 exist, however one of those transcripts is specific to testis. The mouse homolog of ZKSCAN5 is differentially expressed in spermatogenesis. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ZKSCAN5 polyclonal antibody detects endogenous levels of ZKSCAN5 protein. Immunogen :
Synthetic peptide, corresponding to amino acids 284-329 of Human ZKSCAN5. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of ZKSCAN5 polyclonal antibody at 1:500 dilution Lane1:RAW264.7 whole cell lysate(40ug) Lane2:Hela whole cell lysate(40ug) Lane3:H9C2 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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