Product Name :
RHD polyclonal antibody Background :
The Rhesus (Rh) blood group system represents one of the most complex and important systems in humans (1). Two highly homologous genes RHD and RHCE (collectively referred to as RH30 or RHCED) encode the antigens of the Rh blood group system (1–4). These tightly linked genes map to human chromosomal position 1p34.1-1p36 (2,3). The RHD gene, which is commonly deleted from a large segment of the population, encodes the most potent blood group immunogen, the D antigen (2,5). Rh incompatibility between maternal and fetal blood types results in hemolytic disease of the newborn (HDN), which often results in fetal death (1,2,6). The RHCE gene exists in four allelic forms, and each allele determines the expression of two antigens in Ce, ce, cE, or CE combinations (2,4,5). The RHCED antigens exist as integral membrane proteins with contain 12-transmembrane helices, and maintain erythrocyte membrane integrity (2). The presentation of the Rh antigenic activity requires the formation of a complex between the RHCED antigens and RHAG (RH50) (2). Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
RHD polyclonal antibody detects endogenous levels of RHD protein. Immunogen :
Synthetic peptide, corresponding to amino acids 168-213 of Human RHD. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of RHD polyclonal antibody at 1:500 dilution Lane1:The Brain tissue lysate of Rat(30ug) Lane2:The Brain tissue lysate of Mouse(30ug) Lane3:HEK293T whole cell lysate(40ug) Lane4:H9C2 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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