Product Name :
NPAS4 polyclonal antibody Background :
The Per-Arnt-Sim (PAS) domain is a 270 amino acid motif that mediates associations among various PAS family transcription factors. The PAS family contains neuronal specific transcription factors known as NPAS1, NPAS2, NPAS3 and NPAS4, which are involved in the development and maintenance of learning and memory pathways. NPAS1 regulates erythropoietin expression in developing brain. NPAS2, also designated PAS 4/MOP4, associates with MOP3 to activate transcription. NPAS3, which localizes to the nucleus and is ubiquitously expressed in the adult brain, may be involved in neurogenesis and has been implied to control regulatory pathways relevant to psychotic illness and schizophrenia. NPAS4 (neuronal PAS domain protein 4), also known as NXF, Le-PAS, PASD10 or bHLHe79, is a 802 amino acid nuclear protein that is exclusively expressed in the brain. NPAS4 acts as a transcriptional activator in the presence of Arnt 1, and activates the CME (CNS midline enhancer) element and expression of the Drebrin gene. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
NPAS4 polyclonal antibody detects endogenous levels of NPAS4 protein. Immunogen :
A synthetic peptide corresponding to residues in Human NPAS4. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of NPAS4 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:RAW264.7 whole cell lysate

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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