Product Name :
ACOT9 polyclonal antibody Background :
Acyl-CoA thioesterases (ACOTs) are a group of enzymes that catalyze the hydrolysis of acyl-CoA to form coenzyme A (CoA) and a free fatty acid. Through their catalytic activity, ACOTs are able to regulate the level of fatty acids and acyl-CoAs within the cell. ACOT9 (acyl-CoA thioesterase 9), also known as ACATE2, MT-ACT48 (mitochondrial acyl-CoA thioesterase of 48 kDa) or CGI-16, is a 406 amino acid member of the acyl-CoA hydrolase protein family. ACOT9 contains a C-terminal 80-amino acid domain that is conserved from mouse to human, suggesting that the C-terminus may confer the catalytic activity of ACOT9. The gene encoding ACOT9 is located on chromosome X and the expressed ACOT9 protein is localized to the mitochondrion. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ACOT9 polyclonal antibody detects endogenous levels of ACOT9 protein. Immunogen :
A synthetic peptide corresponding to residues in Human ACOT9. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of ACOT9 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:RAW264.7 whole cell lysate Lane3:H9C2 whole cell lysate
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  Immunohistochemistry (IHC) analyzes of ACOT9 pAb bin paraffin-embedded human colon carcinoma tissue at 1:50,showing cytoplasmic staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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