Product Name :
TAF13 polyclonal antibody Background :
TFIID is a general transcription factor that facilitates the preinitiation complex assembly through direct interactions with the TATA promoter element. TFIID is a multisubunit complex consisting of a small TATA-binding polypeptide and other TBP-associated factors (TAFs). The TAF II family members include p18, p28, p32, p100, p130, p170 and p250, which is the largest subunit of TFIID. TAF II p32 is the human homologue of the Drosophila TAFII40 and is upregulated during apoptosis. TAFII p32 interacts with the activation domain of the viral protein 16, TFIIB and the class II transactivator (CIITA) to modulate transcription. The human and murine TAFII p32 proteins are distinct isoforms, designated TAF II p32 a and b, respectively, and are thought to have individual roles in regulation. TAF II p28 and TAF II p18 interact with one another in vitro and intracellularly, and both interact with TBP through distinct domains. TAF II p28 potentiates transactivation of the estrogen and vitamin D3 receptors (ER and VDR), and is the limiting factor in the RXRa activation pathway. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
TAF13 polyclonal antibody detects endogenous levels of TAF13 protein. Immunogen :
A synthetic peptide corresponding to residues in Human TAF13. Conjugate :
Unconjugated Modification :
Unmodification
TAF13 polyclonal antibody Background :
TFIID is a general transcription factor that facilitates the preinitiation complex assembly through direct interactions with the TATA promoter element. TFIID is a multisubunit complex consisting of a small TATA-binding polypeptide and other TBP-associated factors (TAFs). The TAF II family members include p18, p28, p32, p100, p130, p170 and p250, which is the largest subunit of TFIID. TAF II p32 is the human homologue of the Drosophila TAFII40 and is upregulated during apoptosis. TAFII p32 interacts with the activation domain of the viral protein 16, TFIIB and the class II transactivator (CIITA) to modulate transcription. The human and murine TAFII p32 proteins are distinct isoforms, designated TAF II p32 a and b, respectively, and are thought to have individual roles in regulation. TAF II p28 and TAF II p18 interact with one another in vitro and intracellularly, and both interact with TBP through distinct domains. TAF II p28 potentiates transactivation of the estrogen and vitamin D3 receptors (ER and VDR), and is the limiting factor in the RXRa activation pathway. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
TAF13 polyclonal antibody detects endogenous levels of TAF13 protein. Immunogen :
A synthetic peptide corresponding to residues in Human TAF13. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of TAF13 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lys Lane2:H9C2 whole cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide(0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.