Product Name :
TAF1 polyclonal antibody Background :
TAF1L (transcription initiation factor TFIID 210 kDa subunit) is a 1,826 amino acid protein that serves as a functional substitute for TAF II p250 in spermatogenic cells. In somatic cells, TAF II p250, which is located on the X chromosome, is a general transcription factor which initiates preinitiation complex assembly through direct interaction with the TATA promoter element. However, during male meiosis, sex chromosomes are transcriptionally silenced, inactivating the transcriptional activity of TAF II p250. With an autosomal location, TAF1L functionally replaces TAF II p250 in testes. It is documented that the gene encoding human TAF1L arose by retroposition of TAF II p250 mRNA during primate evolution after species divergence. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
TAF1 pAb detects endogenous levels of TAF1 protein. Immunogen :
Synthetic peptide, corresponding Human TAF1. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of TAF1 pAb at 1:500 dilution Lane1:HepG2 whole cell lysate(40ug) Lane2:L02 whole cell lysate(40ug) Lane3:H1792 whole cell lysate(40ug) Lane4:AML-12 whole cell lysate(40ug) Lane5:C6 whole cell lysate(40ug)

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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