Product Name :
APLN polyclonal antibody Background :
Apelin (APEL), an endogenous ligand for APJ, is an alternate coreceptor with CD4 for HIV-1 infection. This secreted protein inhibits HIV-1 entry into cells that coexpress APJ and CD4. By proteolytic processing of the peptide precursor, several different active peptides may be produced. Apelin-36, one such inotropic peptide, is being investigated as a potential plasma marker of cardiopulmonary disease. Apelin is highly expressed in brain, mainly in the thalamus, frontal cortex, hypothalamus and midbrain. Apelin is also secreted by the mammary gland into the colostrum and milk. Oral intake of apelin (in milk and colostrum) may be important in the modulation of the immune responses in neonates and newborns. Apelin has also been found to be a potent stimulator of cardiac contractility and may function in the regulation of the cardiovascular system. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
APLN polyclonal antibody detects endogenous levels of APLN protein. Immunogen :
A synthetic peptide corresponding to residues in Human APLN. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of APLN polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:HELA whole cell lysate

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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