GZMM polyclonal antibody 
Datasheet
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Product Name :
GZMM polyclonal antibody Background :
The granzyme family of proteins belong to the larger peptidase S1 family. Granzyme A and granzyme B are serine proteases that facilitate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Within the granules of activated CTLs, granzyme A and granzyme B are processed and converted to their active forms by the lysosomal cysteine protease cathepsin C. Once cleaved, these active proteases target distinct substrates for proteolysis, thereby mediating apoptosis through two different pathways. Granzyme H localizes to cytoplasmic granules of cytolytic T lymphocytes and is important for target cell lysis in cell-mediated immune responses. Granzyme K is a serine protease localizing to the granules of NK cells and cytotoxic T lymphocytes. Granzyme M (also designated NK cell granular protease, HU-Met-1 or met-1 serine protease) contains one peptidase S1 domain. Granzyme M is a Trypsin-fold serine protease that localizes to granules of large granular lymphocytes (NK cells) and cleaves peptide substrates after leucine, norleucine or methionine. This enzyme may play a role in target cell death induction by cytotoxic lymphocytes. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
GZMM polyclonal antibody detects endogenous levels of GZMM protein. Immunogen :
A synthetic peptide corresponding to residues in Human GZMM. Conjugate :
Unconjugated Modification :
Unmodification
GZMM polyclonal antibody Background :
The granzyme family of proteins belong to the larger peptidase S1 family. Granzyme A and granzyme B are serine proteases that facilitate apoptotic signaling in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Within the granules of activated CTLs, granzyme A and granzyme B are processed and converted to their active forms by the lysosomal cysteine protease cathepsin C. Once cleaved, these active proteases target distinct substrates for proteolysis, thereby mediating apoptosis through two different pathways. Granzyme H localizes to cytoplasmic granules of cytolytic T lymphocytes and is important for target cell lysis in cell-mediated immune responses. Granzyme K is a serine protease localizing to the granules of NK cells and cytotoxic T lymphocytes. Granzyme M (also designated NK cell granular protease, HU-Met-1 or met-1 serine protease) contains one peptidase S1 domain. Granzyme M is a Trypsin-fold serine protease that localizes to granules of large granular lymphocytes (NK cells) and cleaves peptide substrates after leucine, norleucine or methionine. This enzyme may play a role in target cell death induction by cytotoxic lymphocytes. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
GZMM polyclonal antibody detects endogenous levels of GZMM protein. Immunogen :
A synthetic peptide corresponding to residues in Human GZMM. Conjugate :
Unconjugated Modification :
Unmodification
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Western blot (WB) analysis of GZMM polyclonal antibody at 1:500 dilution Line1:Hela whole cell lysate Line2:PC12 whole cell lysate Line3:sp20 whole cell lysate
Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.
Price/Size :
USD 368/1mg/vial
Tips:
For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).Describe :
Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.Formula:
Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.
Storage:
The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C.
Note :
This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.