Product Name :
LY9 polyclonal antibody Background :
T lymphocyte surface antigen Ly9 (CD229), also designated lymphocyte antigen 9 or cell-surface molecule Ly9, is a cell surface glycoprotein. CDC229 is a Type I membrane protein that is crucial in adhesion reactions between T lymphocytes and accessory cells (homophilic interaction). It belongs to the CD2 subfamily of the immunoglobulin gene superfamily of proteins (along with CD2, CD48, CD58, CD84, CD244 and CD150). Receptors of this family are important in cytokine production regulation and cytotoxicity of lymphocytes and NK cells. CD229 interacts with the SAP/SH2D1A protein. CD229 is expressed on mature B cells, T cells, thymocytes and NK cells. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
LY9 polyclonal antibody detects endogenous levels of LY9 protein. Immunogen :
A synthetic peptide corresponding to residues in Human LY9. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of LY9 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:RAW264.7 whole cell lysate Lane3:H9C2 whole cell lysate

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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