Product Name :
ABCA4 polyclonal antibody Background :
The ATP-binding cassette (ABC) superfamily is comprised of transmembrane proteins involved in energy-dependent transport of a variety of substrates across membranes. ABCA4 (also designated ABCR, photoreceptor RIM or RMP) is a photoreceptor specific ATP-binding cassette (ABC) transporter. ABCA4 is exclusively expressed within photoreceptor cells, indicating that ABCA4 mediates the transport of an essential molecule either into or out of photoreceptor cells. Mutations in the gene encoding ABCA4 are responsible for autosomal recessive Stargardt disease (STGD), an early onset macular degeneration, and some forms of autosomal recessive cone-rod dystrophy and autosomal recessive retinitis pigmentosa. In addition, heterozygosity for ABCA4 mutations may possess a risk factor for age-related macular degeneration. ABCA4 is most closely related to the mouse and human ABC1 and ABC2 and maps to human chromosome 1p22.1. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ABCA4 polyclonal antibody detects endogenous levels of ABCA4 protein. Immunogen :
A synthetic peptide corresponding to residues in Human ABCA4. Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of ABCA4 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:PC12 whole cell lysate
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  Immunohistochemistry (IHC) analyzes of ABCA4 pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing membrane staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.
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  Immunohistochemistry (IHC) analyzes of ABCA4 pAb in paraffin-embedded human colon carcinoma tissue at 1:50,showing membrane staining.Negative control (the right)Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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