Product Name :
COL15A1 polyclonal antibody Background :
Collagen Type XV (COL15 gene product) and XVIII (COL18 gene product) form the new subgroup MULTIPLEXIN, within the diverse family of collagens, which contains nineteen distinct types of collagens found in vertebrates. Both Type XV and XVIII collagens are characterized by extensive interruptions in their collagenous sequences. Members of the MULTIPLEXIN subgroup contain polypeptides with multiple triple-helical domains separated and flanked by non-triple-helical regions. Type XV is predominantly expressed in internal organs such as adrenal gland, kidney and pancreas. Type XVIII encodes two different a1 chains, which have different signal peptides and variant N-terminal non-collagenous NC1 domains of 495 and 303 amino acids. The long variant NC1-434 Type XVIII mRNAs are predominantly expressed in liver, while the variant NC1-303 mRNAs are predominantly expressed in heart, kidney, placenta, prostate, ovary, skeletal muscle and small intestine. Endostatin is a fragment of the C-terminal domain NC1 of Collagen Type XV and XVIII that inhibits angiogenesis and tumor growth. Unlike endostatin XVIII, endostatin XV does not bind zinc or heparin, however both endostatins can inhibit chorioallantoic membrane angiogenesis induced by basic FGF or VEGF. Collagen Type XV and XVIII are also widely present in basement membrane zones, suggesting their roles in basement membrane-stromal interactions and involvement with angiogenic and pathological processes. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
COL15A1 polyclonal antibody detects endogenous levels of COL15A1 protein. Immunogen :
A synthetic peptide corresponding to residues in Human COL15A1 Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of COL15A1 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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