Product Name :
ASAP2 polyclonal antibody Background :
DDEF2 (ADP ribosylation factor [ARF]- GTPase-activating protein [GAP] containing SH3, ANK repeats, and PH domain, PAP, PAG3, AMAP1, ZG14P, centaurin beta4) is a phospholipid-dependent ADP-ribosylation factor (ARF) GTPase-activating protein (ARF-GAP) that binds to protein-tyrosine kinases Src and focal adhesion kinase. ARF family GTP-binding proteins are regulators of membrane traffic and cytoskeletal organization. Modulation of ARF activity by DDEF2 is important for the regulation of focal adhesion assembly and/or organization by influencing the mechanisms responsible for the recruitment and organization of focal adhesion proteins paxillin and FAK. In spreading platelets, most endogenous DDEF2 is localized at peripheral focal adhesions. Pyk2 directly phosphorylates DDEF2 on tyrosine-308 and -782, and this event affects the phosphoinositide binding profile of DDEF2. DDEF2 is phosphorylated on tyrosine residues in cells expressing activated Src and tyrosine phosphorylation depends on a proline-rich class II Src SH3 binding site. Product :
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Storage&Stability :
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity :
ASAP2 polyclonal antibody detects endogenous levels of ASAP2 protein. Immunogen :
A synthetic peptide corresponding to residues in Human ASAP2 Conjugate :
Unconjugated Modification :
Unmodification

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  Western blot (WB) analysis of ASAP2 polyclonal antibody at 1:500 dilution Lane1:HEK293T whole cell lysate Lane2:sp2/0 whole cell lysate Lane3:H9C2 whole cell lysate

Bioworld Biotech only provide peptides for our antibodies and do not provide additional peptide customization services.

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Price/Size :

USD 368/1mg/vial

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Tips: 

For phospho antibody, we provide phospho peptide（0.5mg) and non-phospho peptide(0.5mg).

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Describe :

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. This mechanism is useful when non-specific binding is an issue, for example, in Western blotting (WB) and Immunohistochemistry (IHC). By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific; Specific binding will be absent from the western blot or IHC performed with the neutralized antibody.

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Formula:

Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 10 mg/ml.The purity is >90%,tested by HPLC and MS.

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Storage:

The freeze-dried powder is more stable. For short time at 2-8°C. For long term storage store at -20°C. 

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Note :

This product is for research use only (RUO only). Not for use in diagnostic or therapeutic procedures.

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